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產品名稱：重組豬白細胞介素-1受體拮抗劑蛋白蛋白
產品型號：rPoIL-1RA
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組豬白細胞介素-1受體拮抗劑蛋白蛋白與其它公司提供的重組蛋白不同，rPoIL-1RA蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組豬白細胞介素-1受體拮抗劑蛋白蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組豬白細胞介素-1受體拮抗劑蛋白蛋白

Species Porcine

Accession Q29056

GeneID 397499

Source Escherichia coli.

Molecular Weight 重組豬白細胞介素-1受體拮抗劑蛋白蛋白Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ

Purity 重組豬白細胞介素-1受體拮抗劑蛋白蛋白> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 75 pg/ml rPoIL-1α.

Physical Appearance 重組豬白細胞介素-1受體拮抗劑蛋白蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.

Endotoxin Less than 0.1 EU/μg of rPoIL-1RA as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組豬白細胞介素-1受體拮抗劑蛋白蛋白

SDS-PAGE

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Species	Porcine
Accession	Q29056
GeneID	397499
Source	Escherichia coli.
Molecular Weight	重組豬白細胞介素-1受體拮抗劑蛋白蛋白Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ
Purity	重組豬白細胞介素-1受體拮抗劑蛋白蛋白> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 75 pg/ml rPoIL-1α.
Physical Appearance	重組豬白細胞介素-1受體拮抗劑蛋白蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.
Endotoxin	Less than 0.1 EU/μg of rPoIL-1RA as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組豬白細胞介素-1受體拮抗劑蛋白蛋白
SDS-PAGE

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產品名稱：重組豬白細胞介素-1受體拮抗劑蛋白蛋白