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產品名稱：重組人B細胞激活因子/ TNFSF13B蛋白
產品型號：rHuBAFF/TNFSF13B
產品廠商：KALANG
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簡單介紹：

重組人B細胞激活因子/ TNFSF13B蛋白與其它公司提供的重組蛋白不同，rHuBAFF/TNFSF13B蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人B細胞激活因子/ TNFSF13B蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人B細胞激活因子/ TNFSF13B蛋白

Synonyms TNFSF13B, TNFSF20, TALL-1, BLys, THANK, B cell Activating Factor belonging to the TNF family

Species Human

Accession Q9Y275

GeneID 10673

Source Escherichia coli.

Molecular Weight 重組人B細胞激活因子/ TNFSF13B蛋白Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人B細胞激活因子/ TNFSF13B蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a mouse splenocyte survival assay is 0.5-2 μg/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Endotoxin Less than 1 EU/μg of rHuBAFF as determined by LAL method.

Reconstitution 重組人B細胞激活因子/ TNFSF13B蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人B細胞激活因子/ TNFSF13B蛋白

SDS-PAGE

Reference 1. Craxton A, Draves KE, Gruppi A, et al. 2005. J Exp Med, 202: 1363-74.
2. Kalled SL. 2005. Immunol Rev, 204: 43-54.
3. Kuo SH, Yeh PY, Chen LT, et al. 2008. Blood, 112: 2927-34.
4. Rowland SL, Leahy KF, Halverson R, et al. 2010. J Immunol, 185: 4570-81.
5. Liu ZandDavidson A. 2011. Exp Cell Res, 317: 1270-7.

Background B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56 % a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.

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Synonyms	TNFSF13B, TNFSF20, TALL-1, BLys, THANK, B cell Activating Factor belonging to the TNF family
Species	Human
Accession	Q9Y275
GeneID	10673
Source	Escherichia coli.
Molecular Weight	重組人B細胞激活因子/ TNFSF13B蛋白Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人B細胞激活因子/ TNFSF13B蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a mouse splenocyte survival assay is 0.5-2 μg/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
Endotoxin	Less than 1 EU/μg of rHuBAFF as determined by LAL method.
Reconstitution	重組人B細胞激活因子/ TNFSF13B蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人B細胞激活因子/ TNFSF13B蛋白
SDS-PAGE
Reference	1. Craxton A, Draves KE, Gruppi A, et al. 2005. J Exp Med, 202: 1363-74. 2. Kalled SL. 2005. Immunol Rev, 204: 43-54. 3. Kuo SH, Yeh PY, Chen LT, et al. 2008. Blood, 112: 2927-34. 4. Rowland SL, Leahy KF, Halverson R, et al. 2010. J Immunol, 185: 4570-81. 5. Liu ZandDavidson A. 2011. Exp Cell Res, 317: 1270-7.
Background	B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56 % a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.

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產品名稱：重組人B細胞激活因子/ TNFSF13B蛋白