產品詳情
簡單介紹：
重組人B細胞激活因子/ TNFSF13B蛋白與其它公司提供的重組蛋白不同，rHuBAFF/TNFSF13B蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人B細胞激活因子/ TNFSF13B蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人B細胞激活因子/ TNFSF13B蛋白
| Synonyms | TNFSF13B, TNFSF20, TALL-1, BLys, THANK, B cell Activating Factor belonging to the TNF family |
| Species | Human |
| Accession | Q9Y275 |
| GeneID | 10673 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人B細胞激活因子/ TNFSF13B蛋白Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人B細胞激活因子/ TNFSF13B蛋白Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 μg/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
| Endotoxin | Less than 1 EU/μg of rHuBAFF as determined by LAL method. |
| Reconstitution | 重組人B細胞激活因子/ TNFSF13B蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人B細胞激活因子/ TNFSF13B蛋白 | |
| SDS-PAGE |
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| Reference |
1. Craxton A, Draves KE, Gruppi A, et al. 2005. J Exp Med, 202: 1363-74. 2. Kalled SL. 2005. Immunol Rev, 204: 43-54. 3. Kuo SH, Yeh PY, Chen LT, et al. 2008. Blood, 112: 2927-34. 4. Rowland SL, Leahy KF, Halverson R, et al. 2010. J Immunol, 185: 4570-81. 5. Liu ZandDavidson A. 2011. Exp Cell Res, 317: 1270-7. |
| Background | B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56 % a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. |
