產品詳情
簡單介紹：
重組人Interleukin-20蛋白與其它公司提供的重組蛋白不同，rHuIL-20蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人Interleukin-20蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人Interleukin-20蛋白
| Synonyms | Cytokine Zcyto10 |
| Species | Human |
| Accession | Q9NYY1 |
| GeneID | 50604 |
| Source | 重組人Interleukin-20蛋白Escherichia coli. |
| Molecular Weight | Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人Interleukin-20蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose. |
| Endotoxin | Less than 1 EU/μg of rHuIL-20 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人Interleukin-20蛋白 | |
| SDS-PAGE |
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| Reference |
重組人Interleukin-20蛋白1. Sabat R. 2010. Cytokine Growth Factor Rev, 21: 315-24. 2. Blumberg H, Conklin D, Xu WF, et al. 2001. Cell, 104: 9-19. 3. Wei CC, Hsu YH, Li HH, et al. 2006. J Biomed Sci, 13: 601-12. 4. Hsu YH, Li HH, Hsieh MY, et al. 2006. Arthritis Rheum, 54: 2722-33. |
| Background | Human Interleukin-20 (IL-20) is encoded by the IL20 gene located on the chromosome 1 and belongs to the IL-10 family that including IL-10, IL-19, IL-22, IL-24, and IL-26. It is secreted by activated keratinocytes and monocytes, and signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 has functions of regulating proliferation and differentiation of keratinocytes during inflammation, and causing cell expansion of multipotential hematopoietic progenitor cells. |
