產品詳情
簡單介紹：
重組大鼠白細胞介素-33蛋白與其它公司提供的重組蛋白不同，rRtIL-33蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細胞介素-33蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠白細胞介素-33蛋白
| Synonyms | IL-33 |
| Species | Rat |
| Accession | Q66H70 |
| GeneID | 361749 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠白細胞介素-33蛋白Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠白細胞介素-33蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5. |
| Endotoxin | Less than 1 EU/μg of rRtIL-33 as determined by LAL method. |
| Reconstitution | 重組大鼠白細胞介素-33蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠白細胞介素-33蛋白 | |
| Reference |
1. Lingel A, Weiss TM, Niebuhr M, et al. 2009. Structure. 17:1398-410. 2. Baekkevold ES, Roussigne M, Yamanaka T, et al. 2003. Am J Pathol. 163:69-79. 3. Onda H, Kasuya H, Takakura K, et al. 1999. J Cereb Blood Flow Metab. 19:1279-88. 4. Black RA, Kronheim SR, Merriam JE, et al. 1989. J Biol Chem. 264:5323-6. |
| Background | Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Recombinant rat IL-3 contains 156 amino acid residues and it shares 59 % a.a. and 90 % sequence identity with human and murine IL-33. |
