產品詳情
簡單介紹：
重組人角質細胞生長因子1 / FGF-7蛋白與其它公司提供的重組蛋白不同，rHuKGF-1/FGF-7蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人角質細胞生長因子1 / FGF-7蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人角質細胞生長因子1 / FGF-7蛋白
| Synonyms | HBGF-7 |
| Species | Human |
| Accession | P21781 |
| GeneID | 2252 |
| Source | 重組人角質細胞生長因子1 / FGF-7蛋白Escherichia coli. |
| Molecular Weight | Approximately 18.9 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人角質細胞生長因子1 / FGF-7蛋白Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in 20 mM PB, 0.5 M NaCl, pH 8.0. |
| Endotoxin | Less than 1 EU/μg of rHuKGF-1/FGF-7 as determined by LAL method. |
| Reconstitution | 重組人角質細胞生長因子1 / FGF-7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人角質細胞生長因子1 / FGF-7蛋白 | |
| SDS-PAGE |
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| Reference |
1. Mattei MG, deLapeyriere O, Bresnick J, et al. 1995. Mamm Genome. 6:196-7. 2. Kelley MJ, Pech M, Seuanez HN, et al. 1992. Proc Natl Acad Sci U S A. 89:9287-91. 3. de Giorgi V, Sestini S, Massi D, et al. 2007. Dermatol Clin. 25:477-85, vii. 4. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 5. Belleudi F, Leone L, Nobili V, et al. 2007. Traffic. 8:1854-72. 6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700. |
| Background | Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, human KGF-1 shares 96 % amino acid sequence identity with murine, and 92 % with rat. |
